Guide to best okra seed oil CO2 extraction process in 2022

CO2 extraction method

Okra seed→dry at 45℃ to constant weight→crush→supercritical static extraction for 1h→supercritical circulation extraction for 3h→combine the extracts, calculate the extraction rate of okra seed oil→detect the total flavonoid content.

Okra seed oil VE determination method

The content of okraokra seed oil VE was determined by HPLC. The okra seed oil was made into the test solution, and passed through an EP-C18 chromatographic column with methanol as the mobile phase, flow rate 0.3ml/min, injection volume 5μl, column temperature 30 ℃, use UV detector to detect at 285nm, and calculate the content by external standard method.

Get the best okra seed oil co2 extraction process

Temperature of co2 extraction process

In the experiment, under the conditions of 30 MPa and 12.0L/h of CO2 flow rate, the influence of different extraction temperatures (35, 45, 55 ℃) on 300g of dried okra seeds with a crushing particle size of 100 mesh was investigated.

With the increase of extraction temperature, the extraction amount of okra seeds oil showed a logarithmic trend with the increase of extraction time. When the extraction temperature is 45 ℃, the extraction rate of sunflower seeds reaches the maximum.

This is because the increase in temperature will lead to a decrease in the density of the supercritical fluid on the one hand, which is not conducive to the co2 extraction process of solutes; on the other hand, the increase in temperature is conducive to the increase of the mass transfer rate and accelerates the extraction process. Therefore, the appropriate extraction temperature was selected as 45 ℃ in this experiment.

co2 ectractor
co2 ectractor

Pressure of co2 extraction process

The experiment investigated the influence of different extraction pressures (20, 25, 30 MPa) under the conditions of 45°C and a CO2 flow rate of 12.0 L/min.

With the increase of extraction pressure, the extraction volume of okra seeds oil increases logarithmically. When the extraction pressure is 30MPa, the extraction rate of okra seed oil reaches the maximum.

This is due to the increase in pressure. On the one hand, the density of the supercritical fluid increases, which is conducive to the extraction of solute; on the other hand, the increase in pressure is conducive to the increase of mass transfer rate and accelerates the extraction process.

Therefore, this experiment chooses the appropriate extraction temperature to be 30MPa.

small co2 extraction machine for sale
small co2 extraction machine for sale

Time of CO2 extraction process

In the experiment, under the conditions of 30MPa, 45°C and CO2 flow rate of 12.0L/min, the oil output of okra seeds oil in different extraction time periods was investigated.

The oil yield of okraanihot seeds in the first 60 minutes of extraction is high; with the extension of the extraction time, the extraction volume of okra seeds increases logarithmically; after 150 minutes, the oil yield of okra seeds does not increase significantly, so the extraction The rate is increasing slowly.

Therefore, this experiment chooses the appropriate extraction time as 150 min.

Best co2 extraction process

When the CO2 flow rate is 12L/h, the extraction pressure is 30MPa, and the extraction temperature is 45°C, the extraction rate of okra seeds is 16.2%.

In conclusion

Okra is the most edible, medicinal, and health-care function among more than 200 okra plants, and it has high utilization value.

It has broad prospects for its cultivation and development and utilization of active ingredients such as flavonoids.

Through this experiment, we can get:

Using SCF to extract okra oil at an extraction pressure of 30 MPa, an extraction temperature of 45 ℃, and an extraction time of 2.5 hours, the best extraction rate of sunflower oil can be obtained. ;

Under this technological condition, the effective ingredient VE in okra can be extracted, and it is detected to be 23.0mg/100g.
SCF extraction technology can be used for okra oil extraction. A slight improvement in the extraction process can increase the VE yield. If ethanol is added as an entrainer, the effective ingredient flavonoids can be extracted.

The okra seeds oil produced by this process has good solubility and dispersibility, is easily fully absorbed by the human body, has no solvent residue, and is beneficial to health. It is another important extraction technology for okra oil extraction and has application value.

Simply the most complete
CO2 extraction process you can find

CO2 Extraction Process

From raw material preparation, we offer you the most complete CO2 essential oil extraction process.
At the same time, more than 100 CO2 essential oil extraction methods from the laboratory are also provided for you.

MORE

how to extract essential oils

Detailed explanation of CO2 extraction process steps of plant essential oils。

Read More

how to extract cbd

About the co2 extraction machine cannabis.
You can read a lot of articles about cbd oil extraction from the Internet, but I can tell you for sure that they are not comprehensive at all, and even have wrong descriptions.

Let’s start reading

Supercritical CO2 extraction okra seed oil
Supercritical CO2 extraction okra seed oil

Okra

Okra is an annual herbaceous plant of the genus Okra of the Malvaceae family.

The plant height is 1.5-2.0 meters, many branches, dark green leaves, bright yellow flowers 16-18 cm in diameter, and round dark brown seeds.

Medicinal

Okra can be used as both food and anther, which can clear heat and cool blood. The fruit and seeds have the functions of invigorating the spleen and stomach, and promoting muscle growth. It can treat indigestion, reluctance to eat, and traumatic injuries.

Total flavonoids

Its total flavonoids can significantly regulate human endocrine and immunity, increase human resistance, improve cardiovascular and cerebrovascular and microcirculation functions, and enhance the body’s antioxidant capacity; rich natural phytoestrogens are useful for prolonging female puberty and reducing or avoiding middle-aged menopause. Symptoms also have miraculous effects.

Hyperoside

Okra is also rich in hyperoside, and hyperoside has obvious anti-inflammatory effects, strong cough-reducing effect and assimilation effect.

Okra dried flower contains 37.63mg of flavonoids per gram, okra seed kernels contain 26.013mg of flavonoids per gram, okra seed kernel oil contains VE as high as 1.976mg per gram, and the content of flavonoids and VE is dozens of times that of other plants.

HOW TO CHOOSE THE RIGHT CO2 EXTRACTOR?

CO2 Extraction Machine Buyer’s Guide

As you can imagine, not all supercritical CO2 extractors are made the same.
For more than 30 years, we have customized many CO2 extraction machines (Tabletop CO2 Extractor) for many companies and university laboratories (1 lb CO2 extractor). Because each customer’s CO2 extraction process requirements are different, there are almost no identical machines.

How many liters of CO2 extraction vessels are needed?

The first factor that determines the size of the CO2 extraction vessel is the daily working hours of the plant, which determines the daily extraction batch. The vessel size of the CO2 extractor depends on the biomass to be processed per day.

Read More

How many separators are needed?

How can I custom my separator when I need more terpenes in my How can I custom my separators when need more terpenes in CBD oil extraction process?
Waxes separation and Light oil separation in 1st separator;
Light oil separation in 2nd separator;
To trap lightest and volatile compounds in 3rd separator.
It is important to choose the right vessel size that will meet your current needs as well as future demand.

Read More

How many pressure is best for your CO2 extraction process requirements?

The choice of CO2 extraction pressure is a changing factor. If the extraction pressure is too high, the useless substances in the plant will be extracted, such as wax and chlorophyll. If the extraction pressure is too low, the extraction time will be increased.

Read More

How can the minimum operating costs be achieved?

Read More

Further Reading: CBD Oil CO2 Extraction Machine